Electrophoresis nke ọbara na mmamịrị. Electrophoresis nke ọbara na-edozi: ọchịchị transcript

Na plasma nke ọbara mmadụ na e nwere a set nke protein mmiri. Ha na-dị iche iche na ha mejupụtara, Ọdịdị na agagharị na a akpan akpan gburugburu ebe obibi, na-eduzi eletrik. Na nke a mmepụta na nkewa nke ngụkọta protein, nke localizes na plasma maka iche iche protein fractions. Na-eduzi ndị ọbara electrophoresis chọpụta quantitative mmekọrita nke onye protein mmiri na owuwu. Ọ dị mkpa iji chọpụta ma a mmadụ dị iche iche na-enweghị nchịkwa na phenomena, dị ka na-efe efe ma ọ bụ cancer. Ọ bụ protein electrophoresis nke ọbara bụ ihe dị mkpa na-eduzi nchoputa nke ọrịa dị iche iche.

Ihe kachasi mkpa n'ime usoro

N'ihi cleavage protein fractions eji Electrophoresis nke ọbara, ụkpụrụ nke nke dabeere dị iche iche agagharị nke protein mmiri na-ahụ site na electric ubi. Nke a na usoro nke nchoputa bụ ihe ezi na ihe ọmụma, n'adịghị ọkọlọtọ ngụkọta ọbara analysis. Ma nke a electrophoresis egosi naanị a ụfọdụ ọnụ ọgụgụ nke protein fractions, ọdịdị na ruo n'ókè nke enweghị nchịkwa na usoro na a n'ozuzu ụdị. Analysis nke nnyocha na-enye ohere ike ọkachamara iji chọpụta ihe bụ ruru nke protein fractions hụrụ ụmụ mmadụ, na iji chọpụta kpọmkwem nke daa ọrịa pụta ụwa a akpan akpan ọrịa.

Types of protein fractions

Ọtụtụ n'ime ndị isi mmadụ ndu ọmụmụ ma ọ bụ ọbara, bụ edozi. Ngụkọta ego nke norm bụ n'ime 60-80 g / l. N'ihi na ezi analysis na electrophoresis nke ọbara na akwụkwọ. Nke a na-amụ bụ ihe kasị nkịtị usoro nke analysis. Isi usoro bụ a pụrụ iche nyo akwụkwọ. Ya isi mma - elu absorbability. Ụdị akwụkwọ ahụ nwere ike banye mmiri karịa arọ ya na 130-200 ugboro. Dabere na akụrụngwa na-eji electrophoresis na akwụkwọ dịruru 4-16 awa. Ọ na-aga unit nke protein owuwu. Mgbe ahụ, akwụkwọ akụkọ warara-emeso ya na pụrụ iche ink maka analysis. Nke a na Usoro bụ ihe kasị nkịtị na ahụ ike na laboratories. N'ihi mmetụta nke eletrik protein nta, na-ezighị ezi ebubo, na-akpụ akpụ n'ebe ghaghị ebubo electrode. N'ihi na protein mmiri nke ọbara na-ekewa n'ime 5 fractions mara:

• albumin;
• α ₁ globulins;
• α ₂ globulin;
• β - globulin;
• γ-globulin.

Albumins na-ezighị-ebubo, na-obere tụnyere ndị ọzọ ụmụ irighiri ihe, molekụla arọ. N'ihi na nke a ije ọsọ nnọọ elu karịa ndị ọzọ nke òtù ha, ha na-emi odude furthest si malite ebe. Atọ ndị mbụ ụmụ irighiri ihe globulin aga na a iji nwayọọ na-agba n'ihi na nke arọ. Ma ndị kasị nta na-agba na-dere na γ-globulins. Ndị a na-edozi na-arọ na nnukwu, ikwu na ndị ọzọ akụkụ. Ha ụgwọ bụ fọrọ nke nta na-anọpụ iche, otú a protein nta na-adịghị gbanwere ka na-amalite akara.

mkpa iji

Ugbu a, electrophoresis nke ọbara nyochara na-mụụrụ maka ezi nchoputa nke ọrịa. Nke a analysis nwere ike e nyere dị ka a therapist, otú dọkịta warara profaịlụ. Egosi ga-na Research:

• dị iche iche na mbufụt;
• na-adịghị ala ala ọdịdị nke ọrịa;
• enweghị nchịkwa Filiks ke connective anụ ahụ;
• ọbara ọgbụgba;
• ịza aza neoplasms.

Nkwadebe maka nnyefe nke analysis

Iji na-amụ omume nke ndị a pụta ziri ezi, ọ dịkarịa ala 8 awa tupu ọbara onyinye dị mkpa inye elu-eri oriri. Ke adianade do, ọ dị mkpa na-ekweta na-ewere ọgwụ, ma ọ bụrụ na ọ bụla, na ịga dibịa.

Nlele ọbara

Maka pụta nọ na-ezighị overstated, ọ dị mkpa na-ebelata-ekwe omume nke ọbara clotting kpebie a protein fractions na ngụkọta protein. Electrophoresis ọbara ọbara a rụrụ nke ọma, dị ka e nwere a likelihood nke distortion nke pụta n'ihi fibrinogen. Ọ nwere ike zoo ndiiche na-edozi ma ọ bụ na-mgbagwoju anya na ha.

nkịtị ụkpụrụ

N'ime ụbọchị mgbe ụbọchị ndị sample dị njikere ịnwale maka electrophoresis nke ọbara na-edozi. Norma natara arụmọrụ ige okenye:

1. Total protein - 63-82 g / l.
2. Albumin - 40-60% nke ngụkọta ọnụ ọgụgụ nke ụmụ irighiri ihe.
3. α-globulins ₁ - 2-5%.
4. α-globulins ₂ - 7-13%.
5. β-globulins - 8-15%
6. γ-globulins - 12-22%.

Mkpa iji nyochaa

Na-agbanwe agbanwe ego nke ọ bụla protein nta na elu ma ọ bụ ala nwere ike na-egosi na mmepe nke a akpan akpan daa ọrịa. Iji nweta eziokwu banyere nke a kwesịrị electrophoresis nke ọbara na-edozi. Nyopụta pụta ikwado ọgwụ ọkachamara nchoputa na oke nke ọgwụgwọ.

The abawanye na albumin

Na mbu, chọpụta ego nke albumin na analysis nke pụta. The abawanye na a nta ike ikwu banyere akpịrị ịkpọ nkụ. Nke a nwere ike ime ma ọ bụrụ na onye ọrịa ahụ na a ruo ogologo oge vomiting ma ọ bụ ọrịa na digestive usoro. Albumin na-amụbakwa n'akparamagwa Burns a nnukwu ebe nke anụ.

belata albumin

Ọtụtụ ihe ndị ọzọ dị ize ndụ, ma ọ bụrụ na na ahu mbelata ego nke albumin, o nwere ike na-ekwu okwu ndị na-esonụ pathologies:

1. Ọrịa nke akụrụ na imeju.
2. Pathology nke eriri afọ tract.
3. Infectious Filiks.
4. -Enwe nsogbu obi usoro.
5. Ọbara ọgbụgba.
6. Malignancies.
7. Sepsis.
8. Rheumatism.

A nta ọnụ ke ego nke albumin nwekwara ike ịbụ:

1. Na abụ nne.
2. Na ngafe doses nke ọgwụ ọjọọ.
3. N'oge ruo ogologo oge fever.
4. Na arọ na-ese siga.

Na-agbanwe agbanwe ọnụ ọgụgụ nke α1-globulins

Mbenata ego nke A1-globulins dere na a erughi nke α ₁ -antitrypsin. Ịba otu ebe na exacerbation nke mbufụt ke idem, ọrịa imeju na anụ ahụ na-ala.

Mbenata α2-globulins

Deba aha ya na-arịa ọrịa shuga, mbufụt nke pancreas, a mụrụ ọhụrụ na jaundice na ịba ọcha n'anya na-egbu egbu si malite. Ọ na-egosi na-ekwesịghị ịdị, ekwesịghị nri.

The abawanye na α2-globulins

Ọ na-adị n'okpuru ndị na-esonụ ọnọdụ:

1. Inflammations, karịsịa na ọnụnọ nke purulent exudates (akpa ume mbufụt na ndị ọzọ na Filiks na ọnụnọ nke abu).
2. Connective anụ ahụ ọrịa (e.g., rheumatic ọrịa).
3. Malignancies.
4. Oge nke mgbake mgbe Burns.
5. Akụrụ aka.

Ke adianade do, ndị dị otú ahụ a onu e ji mara nke ọbara hemolysis na vitro n'oge ọmụmụ.

Mụbara β-globulins

Hyperlipidemia gosiri na (na-amụba ego nke lipids na ọbara), imeju na-na akụrụ daa ọrịa. Nwere ike achọpụtara na na-emeghe afo ọnyá afọ, na hypothyroidism (thyroid aghara ọrụ). Belata fractions na-ekwu na gipobetalipoproteinemii (abawanye na mejupụtara ọbara betalipoprotein).

Mgbanwe ndị na-akụkụ dị nta nke γ-globulins

Nke a òtù na-agụnye ya otu immunoglobulins. Ya mere na-abawanye na γ-globulins dere na ọdịda na ọgụ. Nke a na-emekarị na-adị mgbe a dịgasị iche iche nke na-efe efe, na mmepe nke mkpali usoro, na-agbanwe na anụ ahụ na ọkụ unan. The uto nke γ-globulins na hụrụ ọrịa na-adịghị ala ala ịba ọcha n'anya. Fọrọ nke nta otu ọnọdụ e ji mara nke imeju. Na oké ikpe nke ọrịa a ego nke protein γ-globulin nta budata elu karịa albumin. N'okpuru ọrịa ụfọdụ nwere ike ida ke kpụworo γ-globulins, na bụ mmepe nke gbanwetụrụ na-edozi na ọbara - paraprotein. immunoelectrophoresis - n'ihu ọmụmụ a na-eme iji chọpụta ọdịdị nke onye ahụ a development. Ụkpụrụ a bụ ahụkarị myeloma Waldenstrom ọrịa na daa ọrịa.

Amụba ego nke γ-globulins nwekwara pụta ụwa na-esonụ pathologies:

• lupus erythematosus;
• endotelioma;
• etolite ọrịa ogbu ogbu na nkwonkwo;
• osteosarcoma;
• adịghị ala ala lymphocytic leukemia;
• candidiasis.

Mbenata ego nke γ-globulins

The iju ke γ-globulins na-ekewa n'ime 3 ụdị:

1. Saline (ahụkarị ụmụ atọ na ọnwa ise).
2. Congenital (-emepe emepe mgbe amuchara nwa).
3. Mgbawa (mgbe ihe na-akpata mmepe ike ike ike).

Secondary ọnụ aha na mmepe nke ọrịa na-eme ka depletion nke dịghịzi usoro. Nso nso a na ọgwụ na ahụ ike omume bụ esiwanye ihe analysis chọpụta ukwu nke prealbumin. A, ndị dị otú ahụ a na-amụ rụrụ ndị ọrịa na ọzụzụ kpụ ọkụ n'ọnụ na-elekọta.

Mbenata ọnụ ọgụgụ nke prealbumin dị ezigbo mkpa na ezi ule iji chọpụta owuwu nke protein erughi ke idem nke onye ọrịa. N'oge analysis rụrụ prealbumin mgbazi protein metabolism na ndị ọrịa a.

mmamịrị electrophoresis

Ụkpụrụ nke ihe dị otú ahụ analysis yiri n'ịrụ electrophoresis nke ọbara technology. Ọ na-rụrụ maka ihe ezi nchoputa ma ọ bụ nchọpụta nke ndị ọzọ abnormalities. Ke adianade do, ihe dị otú ahụ analysis ga-enyere iji mata ọrịa ọnụnọ nke proteinuria.

ọgwụgwụ

Electrophoresis nke ọbara na mmamịrị - mkpa ụzọ na nchoputa nke dị iche iche ọrịa. N'ihi nnyocha ụkpụrụ na izi ezi, ha na-enyere iji chọpụta ụdị daa ọrịa. Ezi nchoputa - a n'aka ụzọ dozie ọgwụgwọ na zuru mgbake.