Fluorescent Microscopy: atụmatụ research

Okwu ahụ bụ "mikroskopu" nwere Greek mgbọrọgwụ. Na translation ọ pụtara ọmụmụ ihe na elu-nkenke ngwá. . Na-adịbeghị anya na-esiwanye na-ewu ewu fluorescent na elektrọn Microscopy.

mkpebi

N'okpuru ya, dị ka a na-achị, na-aghọta na kacha nta anya na nke nwere ike n'ụzọ doro anya distinguishable akpọkwa. Na mkpebi ike nke ngwá ogo penetration ga-adabere na microscopic ụwa, pụrụ ịhụ size of ihe mmewere. Na a nnukwu abawanye na ókè-ala ụlọ ọrụ nwere ike jikota. Ntem, e nwere ndị ụfọdụ ókè, n'ofè nke obibia nke ndị ọcha bụ isi.

The usoro nke fluorescence Microscopy: usoro

Mgbe ume akakabarede nke na-amịkọrọ umi n'ime anya radieshon emee egbukepụ egbukepụ. Ọ na-akpọ luminescence. Nke a na onu bụ n'ihi na eziokwu na ụfọdụ bekee n'okpuru nduzi nke ìhè amalite emit ụzarị si ọzọ (na-emekarị nnukwu) wavelength. Ọzọkwa, ụfọdụ ihe ndị na-na nkịtị ọkụ ọnọdụ kpọmkwem agba n'okpuru nduzi nke ultraviolet ìhè na-agbanwe ya na agba.

Specificity

Ihe a na-apụghị hụrụ n'okpuru UV ìhè, ike emit na-egbuke egbuke luster, ọ bụrụ na ọ na-emeso ya na a pụrụ iche umi. Na ya ọcha nke dị iche iche na agba nke ìhè na ọchịchịrị. radiant osisi ike dị iche iche, ma dị ka a na-achị, ọ bụ obere. Na nke a, fluorescence Microscopy bụ irè a na-agba ọchịchịrị na ụlọ. Mgbe na-eji ụdị nyocha a nwere ike hụrụ na ìhè nke ihe, nke o ọchị. The chemical mejupụtara nke anụ ahụ, na mkpụrụ ndụ ga-emetụta àgwà nke na-amụ ihe. Fluorescence Microscopy na-atụle a ruo n'ókè ụfọdụ histochemical ọmụmụ.

nhazi ọkwa

может быть первичной или вторичной. Fluorescence Microscopy pụrụ isi ma ọ bụ nke abụọ. Na nke ikpeazụ ahụ, ihe na-esichara site pụrụ iche ogige enye luminescence. основывается на собственной способности исследуемого элемента излучать свет. Primary fluorescent Microscopy dabeere na ha onwe ha ike nke ihe mmewere emit ìhè.

akụrụngwa

Fluorescence Microscopy a rụrụ iji a dịgasị iche iche nke ngwaọrụ. The illuminator eje ozi dị ka isi nke mmewere. Ọ na-onwem na UV oriọna. Ke adianade do, na ngwaọrụ na-eji a set nke nzacha. Na ụfọdụ ngwá bụ nnọọ ole na ole dị iche iche configurations. Dabere na nke na agba na-eji maka luminescence excitation - ultraviolet ma ọ bụ-acha anụnụ anụnụ, n'etiti ìhè isi ihe na ihe ahụ n'okpuru nchoputa-etinye kwesịrị ekwesị iyo. Ebe ọ bụ na luminescence microscopic mmewere energetically ọgwụgwụ excitation ìhè, ọ ga-ejide naanị otu ihe. Njuputa nke ụzarị inyịme si iyi na-ebipụ odo-green iyo. Ọ na-etinye na ngwá eyepiece. The kasị akpọ mmetụta luminescence ga-eme mgbe nyo na-ebechapu ya kpamkpam ka ụzarị ìhè oku si iyi.

Gịnị bụ echichi na anya ìhè? Na ya na e nwere a na-egbuke egbuke ìhè isi na a ndu mikroskopu. N'etiti mirror unit na a oriọna n'ịnyịnya-acha anụnụ anụnụ-violet iyo. Nke a pụrụ ịbụ FS-1, UFS-3 na na. Yellow nyo dabara n'elu eyepiece nke mikroskopu. Na enyemaka ha, ihe na-ada acha anụnụ anụnụ-violet ìhè. Ọ obi ụtọ luminescence. Ma nke a ìhè nwere ike igbochi ya na ngosi ọkụ. Ya mere, na ụzọ anya ọ ebipuworo a odo iyo. Nwụnye nke ọkụ mere Koehler usoro, na otu dị iche. Diaphragm condenser ga-n'ụzọ zuru ezu na-emeghe. Na-amụ, ọ dị mkpa iji na-abụghị fluorescent imikpu mmanụ. Iji belata intrinsic emission na kwukwara thereto nitrobenzene (2-10 tụlee / 1 g).

Fluorescent Microscopy: ngwa ke microbiology

The uru nke ụdị nyocha a bụ:

1. Ekwe omume inweta agba oyiyi.
2. High iche onwe-enye ìhè ọcha na nwa ndabere.
3. Nchọpụta na orunótu nke ụdị ụfọdụ nke nje na germs.
4. Ekwe omume nke na-amụ uzo na opaque ndụ ntule.
5. The ọmụmụ nke ndụ Filiks na ha Ọnọdụ.

Ọ dịkwa mkpa ka na-ekwu na fluorescent Microscopy akwalite ndị kasị mma ụzọ nke histology na cytochemistry, ngwa ngwa nchoputa.